Plasma fractionation is the separation of plasma proteins based on their physical and chemical properties such as solubility, size, and charge.
The goal is to isolate therapeutic proteins like:

Albumin – for treating shock and burns
Immunoglobulins (IgG) – for immune deficiencies and autoimmune diseases
Coagulation factors – like Factor VIII and IX for treating hemophilia

Methods of Plasma Fractionation
Cohn Process (Cold Ethanol Fractionation)

• Developed by Edwin J. Cohn in the 1940s
• Uses cold ethanol at varying pH and ionic strength to precipitate proteins
• Still widely used today

• More precise separation using resins and columns
• Types include ion-exchange, affinity, and size-exclusion chromatography
• Used in modern fractionation facilities

• For concentrating and purifying proteins
• Often used with other methods